This work was supported by Management & Science University (MSU) under Grant [MSU-RMC-02/FR01/08/L3/***] and the company  providing ZACA extracts (wonderdrink6).

In this research, wonderdrink6 is called ZACA extracts. It’s an abbreviation form of Zinger officinale(Ginger), Allium sativum(Garlic), Citrus(Lemon), honey, and Malus domestica (Apple Cider)vinegar.

Kokila Vani Perumal1, 2 , Kasturi Kanniappan2 , Hasnah Bahari1, and Santhra Segaran Balan1

6 groups of rats were put into research and observed by twice a day basis. Person in charge cleans the containers on daily basis, clearing off the leftover and faeces.

N.C. -  Normal chow Diet (this is a reference group)

P.C. - High Cholesterol Diet

D.C. - High Cholesterol Diet, Simvastatin Drug control

W1 - High Cholesterol Diet, Consuming wonderdrink6  1mg per kg, Or equivalent to 6ml For human

W2 - High Cholesterol Diet, Consuming wonderdrink6  3mg per kg, Or equivalent to 18ml For human

W3 -  High Cholesterol Diet, Consuming wonderdrink6  5mg per kg, Or equivalent to 30ml For human

During an informal discussion between MSU and Natural Tone, we found out there is nothing changed in rats from the vivo: the only difference is group NC (normal chow diet) rats are slimmer.

At the end of the study, the rats were fasting for 12 hours. Thereafter, the rats were anesthetized with diethyl ester. The blood samples were obtained by using the cardiac puncture method. Then, the blood samples were centrifuged (3000 rpm for 15 minutes) to separate the serum. The serum (supernatant) was removed and stored at -20°C for biochemical assay. Liver, kidney, retroperitoneal adipose tissue (RpWAT), visceral fat, gonadal fat were removed, washed with saline, and weighed. The parts of the liver, kidney, RpWAT tissue were fixed in 10% NBF (neutral buffered formalin) for histological examination.

Based on vitro data, ZACA extracts had ORAC value 2000 μmol TE/100 mL, TPC (7537 ± 54.5%), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (27.34 ± 2.71%) , Elastase inhibitory assay (29.29 ± 1.65%) and Lipoxygenase inhibitory assay (98.58 ± 1.42%). Based on vivo data, ZACA extracts treated groups reduced in bodyweight, organ weights (Liver, Adipose tissue, Kidney) and positive improvement in blood toxicity (Creatinine, AST, ALT) and lipid profile (TC, TG, LDL, HDL). 

In conclusion: The results demonstrate that ZACA extracts have a hyperlipidemic activity that can be an alternative approach to combat hyperlipidemia complications

Management and Science University (MSU) participate in virtual Malaysia Technology Expo (MTE2021), Malaysia. This Hyperlipidaemia research has won GOLD Medal in healthcare, personal care, biotechnology and life sciences category.

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